Journal: BMC Genomics

Article Title: JMJD3 regulate H3K27me3 modification via interacting directly with TET1 to affect spermatogonia self-renewal and proliferation

doi: 10.1186/s12864-024-10120-9

Figure Lengend Snippet: Primer of PCR

Article Snippet: Proteins were analyzed with anti-β-actin (1:10000, 66009-1-Ig, Proteintech), anti-EZH2 (1:1000, 21800-1-AP, Proteintech), anti-JMJD3 (1:1000, A01309, BOSTER), anti-H3 (1;1000, A12477-2, BOSTER), anti-H3K27me3 (1:1000, #9733, CST), anti-PCNA (1:1000, BS6438, Bioworld), anti-DAZL (1:1000, A02069-2, BOSTER), anti-GFRA1(1:1000, PB0199, BOSTER) anti-AKT (1:1000, T55561, Abmart), anti-P-AKT (1;1000, T40067, Abmart).

Techniques:

Journal: BMC Genomics

Article Title: JMJD3 regulate H3K27me3 modification via interacting directly with TET1 to affect spermatogonia self-renewal and proliferation

doi: 10.1186/s12864-024-10120-9

Figure Lengend Snippet: Effects of EZH2 interference or overexpression and JMJD3 interference or overexpression on self-renewal, proliferation and differentiation of spermatogonia. ( A ) The mRNA levels of PCNA, Cyclin-A, GFRA1, PLZF and C-KIT related to spermatogonia self-renewal and proliferation were changed after EZH2 and JMJD3 knockdown. ( B ) The expression of PCNA, cyclin-A, GFRA1, PLZF, C-KIT, DAZL and VASA was detected by qRT-PCR after EZH2 and JMJD3 overexpression. ( C ) The protein expression changes as well as statistical analysis of PCNA, DAZL and GFRA1 after EZH2 and JMJD3 overexpression. The membrane is lysed prior to hybridization with the antibody and the image has been cropped for a more aesthetically pleasing display. The full- length blots can be obtained from Additional file 2: Fig . ( D ) The cell cycle of EZH2 and JMJD3 overexpression cells was detected by flow cytometry. ( E ) Protein interaction network of EZH2, JMJD3 and spermatogonia self-renewal, proliferation and differentiation-related genes

Article Snippet: Proteins were analyzed with anti-β-actin (1:10000, 66009-1-Ig, Proteintech), anti-EZH2 (1:1000, 21800-1-AP, Proteintech), anti-JMJD3 (1:1000, A01309, BOSTER), anti-H3 (1;1000, A12477-2, BOSTER), anti-H3K27me3 (1:1000, #9733, CST), anti-PCNA (1:1000, BS6438, Bioworld), anti-DAZL (1:1000, A02069-2, BOSTER), anti-GFRA1(1:1000, PB0199, BOSTER) anti-AKT (1:1000, T55561, Abmart), anti-P-AKT (1;1000, T40067, Abmart).

Techniques: Over Expression, Knockdown, Expressing, Quantitative RT-PCR, Membrane, Hybridization, Flow Cytometry